To make this Southern Five Layer Beef Casserole gluten-free. You'll need to ensure. That all your ingredients are certified gluten-free. Especially the ground beef. Some ground beef preparations might include additives. Or fillers that contain gluten. So it’s important to read labels carefully. Or consult with your butcher. Additionally, when greasing your baking dish. Use a gluten-free cooking spray. Or a butter substitute. That’s labeled gluten-free. This small adjustment. Will allow you to enjoy this cozy, comforting dish. Without any gluten concerns. Enjoy your gluten-free culinary creation. With the same rich flavors. And hearty textures!