To make these Peanut Butter and Jelly Cupcakes gluten-free, replace the all-purpose flour with a certified gluten-free 1:1 baking flour blend. Confirm that the peanut butter, jam or jelly, roasted peanuts, baking soda, baking powder, cream cheese, confectioners’ sugar, vanilla extract, sour cream, heavy cream, butter, and all packaged ingredients are labeled gluten-free. Check roasted peanuts and jelly carefully, as some brands may have cross-contact warnings or gluten-containing additives. Use clean mixing bowls, measuring tools, muffin pans, liners, piping bags, decorating tips, spatulas, and cooling racks to prevent gluten contamination.