To make this Cowboy Cornbread Trifle gluten free, use a certified gluten-free cornbread mix or a homemade gluten-free cornbread recipe in place of the boxed mix. Also verify that the ranch dressing, mayonnaise, chili beans, bacon, and smoked cheddar are certified gluten free or free from cross-contact risks, as packaged products can vary by brand. The rest of the ingredients are typically naturally gluten free, but label-checking is especially important when preparing this dish for someone with celiac disease or gluten sensitivity.